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Biofuel Cans are created when Empty Cans are put into the can slot of a Still. The Still will then create Biofuel Cans and deposit them in its manual input/output slot. Using Wax Capsules will produce functionally identical Biofuel Capsules.

Both types can be put into the manual input/output slots of Bottler Plants and BioPower Generators to fill those machine's resource tanks. One Biofuel Can in a BioPower generator will last for 60 seconds and will produce 16 EU/t thus making a total output of 19,200 EU.